Soluble DHFR expression ranges in molecules per mobile measured from lysate activity assays as described in resources and methods.
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To test this prediction, we reintroduced chromosomal Lon expression underneath the control of a constitutive promoter inside our collection pressure, and repeated deep mutational scanning in biological triplicate (Supplementary file two). We check with the two regimes as +Lon and –Lon range. the standard of +Lon variety was similar to that of –Lon collection (determine two—figure nutritional supplement 1, determine 2—figure complement 2). according to our hypothesis, the distribution of variety coefficients shifted to a lot more damaging values within the +Lon assortment, depleting positive range coefficients and enriching for damaging or null coefficients (Figure 2A).
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